Insertion junction: LMJ.RY0402.133856_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.133856
Chromosome:chromosome 7
Location:1587650
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g324800 SNE6 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGAGCGGCTGCTGAGGGCTGGCCACACG
Internal bar code:TTTGTGCGATTAAGGTGGTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:955
LEAP-Seq percent confirming:99.8106
LEAP-Seq n confirming:2108
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCCTAAACTCTCGCTGC
Suggested primer 2:TGCCATGTGGATGACGTAGT