| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133867 |
| Chromosome: | chromosome 7 |
| Location: | 5028743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347200 | (1 of 1) 1.8.4.14 - L-methionine (R)-S-oxide reductase / FRMsr | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGACGGCCTCTTGTCTCTGGCATGAC |
| Internal bar code: | GAAACTGGATGTACGAGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 605 |
| LEAP-Seq percent confirming: | 99.5569 |
| LEAP-Seq n confirming: | 19546 |
| LEAP-Seq n nonconfirming: | 87 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGTGGCTGGGAGATCAT |
| Suggested primer 2: | AAGCGTGCTGTGCTATGATG |