| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133884 |
| Chromosome: | scaffold 19 |
| Location: | 74471 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750547 | NDA2 | thylakoid NADH dehydrogenase; (1 of 3) K17871 - NADH:ubiquinone reductase (non-electrogenic) (ndh1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCAAGGTGGGGAGGAGCAGGGAGGAGA |
| Internal bar code: | TGACCTCTGCCTAATCTTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 55.3191 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAGCTGCTCGGCATAGT |
| Suggested primer 2: | GAAGGAGGAGGGAACAGGAC |