Insertion junction: LMJ.RY0402.133925_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.133925
Chromosome:chromosome 10
Location:979622
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC 3'UTR|intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCTGTGATGCACGGAACCATCAGCAGC
Internal bar code:TTCTCGTGGCACTAGGGCATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:327
LEAP-Seq percent confirming:99.64
LEAP-Seq n confirming:20761
LEAP-Seq n nonconfirming:75
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACATACACACGCACCC
Suggested primer 2:GTGTGTCGTGAGGTTCCCTT