| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133927 |
| Chromosome: | chromosome 5 |
| Location: | 362038 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233305 | (1 of 1) K07071 - uncharacterized protein (K07071) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGGGTGAAGAGCTTTGAGCGCTGGGA |
| Internal bar code: | GGGCGTGGTGCTCTAACTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 70.7865 |
| LEAP-Seq n confirming: | 126 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACCTACAGCGAAGACTC |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |