Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.133947 |
Chromosome: | chromosome_3 |
Location: | 7553512 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g205050 | DGTT4,CGLD24,DOT1 | Diacylglycerol O-acyltransferase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGGGATTGGAATACGCTACCTCCCCCGCT |
Internal bar code: | ACAGTCCGTACTTGGCCGGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 979 |
LEAP-Seq percent confirming: | 97.9667 |
LEAP-Seq n confirming: | 6649 |
LEAP-Seq n nonconfirming: | 138 |
LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACTTGTTCGGATTCTGCCC |
Suggested primer 2: | GCAGTGGCAGTAAAACAGCA |