| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133957 |
| Chromosome: | chromosome 8 |
| Location: | 4557428 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g382825 | (1 of 2) 2.7.7.49//2.7.7.7//3.1.26.4 - RNA-directed DNA polymerase / Revertase // DNA-directed DNA polymerase / DNA-dependent DNA polymerase // Ribonuclease H / RNase H | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCTGAGGTGTGATGCTTGGTGGGGCGA |
| Internal bar code: | GTTTGTTGAAATAAATAGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 511 |
| LEAP-Seq percent confirming: | 99.3946 |
| LEAP-Seq n confirming: | 2955 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGCTTGGGTATTCTTCC |
| Suggested primer 2: | CAGGGACACACACACACACA |