| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.133958 |
| Chromosome: | chromosome 10 |
| Location: | 6546739 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g467100 | (1 of 1) PTHR31633//PTHR31633:SF1 - FAMILY NOT NAMED // H/ACA RIBONUCLEOPROTEIN COMPLEX NON-CORE SUBUNIT NAF1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGTGACAGCGCGGAGGACAGCAGTGAG |
| Internal bar code: | CAGCATACTAAGCCGGTGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 40 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 87 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGAGGGGGTGTAAAGG |
| Suggested primer 2: | GTAACGCTGGAGTCGTGTCA |