Insertion junction: LMJ.RY0402.133966_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.133966
Chromosome:chromosome 9
Location:1237781
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g399601 (1 of 15) IPR029057 - Phosphoribosyltransferase-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTTACTGCGCCAGAACCGGTAAACTACC
Internal bar code:TGCTCGCCCGCGAAGATGGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:50
LEAP-Seq percent confirming:97.6744
LEAP-Seq n confirming:168
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGGAGCGCATAACAGAA
Suggested primer 2:GCAGTATCGCTCGAGTTTCC