| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.133975 |
| Chromosome: | chromosome 2 |
| Location: | 2784096 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094150 | HIK,HIK2,HKR2 | (1 of 1) K12130 - pseudo-response regulator 5 (PRR5); Response regulator of two-component system | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCACACGGTGGCAGAGCTAGCTTTGGT |
| Internal bar code: | TCACTGTTTCTAACTTAACCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 58.4428 |
| LEAP-Seq n confirming: | 4714 |
| LEAP-Seq n nonconfirming: | 3352 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTAGCAATCGCGGAAGAC |
| Suggested primer 2: | CCGCCGTATTTAAAAGGGTT |