| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134015 |
| Chromosome: | chromosome 6 |
| Location: | 2127773 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265602 | (1 of 1) IPR006569//IPR008942//IPR009010 - CID domain // ENTH/VHS // Aspartate decarboxylase-like domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGGACCGGCGCTCCATTGTGACGCTCAA |
| Internal bar code: | CCATAGCGAACTAGTTGGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 98.1395 |
| LEAP-Seq n confirming: | 422 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCTCCAGTAATGCCCGA |
| Suggested primer 2: | TTGCACGGTAGCAGTAGTGG |