| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134029 |
| Chromosome: | chromosome 2 |
| Location: | 5704358 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110050 | (1 of 1) PTHR22844:SF160 - WD40 REPEAT-CONTAINING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGGAAACGTAAGGATGGCCTGGCATGC |
| Internal bar code: | CGTCGAGCGGCCAATGAGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 99.4106 |
| LEAP-Seq n confirming: | 1012 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGACACGTGGACACCATC |
| Suggested primer 2: | CATACGTACCTCCTTGCCGT |