| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134049 |
| Chromosome: | chromosome 2 |
| Location: | 3744481 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095250 | (1 of 1) 6.6.1.2 - Cobaltochelatase / Hydrogenobyrinic acid a,c-diamide cobaltochelatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCTGAGAAGCAGCAGCATGCTGAGGAG |
| Internal bar code: | TCACGGATCCGCAGCGGCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 99.6118 |
| LEAP-Seq n confirming: | 5645 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCATGCTGCTGCTATCAC |
| Suggested primer 2: | CATTGTTAGCATCCACCACG |