| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134091 |
| Chromosome: | chromosome 12 |
| Location: | 3715448 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514700 | PPHC1,PPH | Pheophytinase 1, chloroplastic; (1 of 1) PTHR10992:SF879 - PHEOPHYTINASE, CHLOROPLASTIC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAACCGCCGCCGCCTCCATCACCTCCCTG |
| Internal bar code: | ACGTTAGGAGGCCTCAGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 685 |
| LEAP-Seq percent confirming: | 99.3159 |
| LEAP-Seq n confirming: | 2323 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTGTGCTGTTCCTGATA |
| Suggested primer 2: | TAGGGTGCACCAGCATAACA |