Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.134112 |
Chromosome: | chromosome_11 |
Location: | 411484 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre11.g467582 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGGCGAGCGCCCCGAGCTTCCGCGTTACG |
Internal bar code: | CTTGTGATGTATTTCCGTTGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 182 |
LEAP-Seq percent confirming: | 39.0625 |
LEAP-Seq n confirming: | 25 |
LEAP-Seq n nonconfirming: | 39 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACACAGTACCCAAAAGCCG |
Suggested primer 2: | TGCAGTATGCAATGGTGGTT |