| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134122 |
| Chromosome: | chromosome 1 |
| Location: | 3472520 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022100 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTAGCGCGAAGCGTGTGTCGCGTGTG |
| Internal bar code: | GGCGGGTGAATGGTAAAGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 97.648 |
| LEAP-Seq n confirming: | 4650 |
| LEAP-Seq n nonconfirming: | 112 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGACTGGCTACCTCCCT |
| Suggested primer 2: | CGGCGTGGGATGTAACTAAG |