| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134135 |
| Chromosome: | chromosome 13 |
| Location: | 3587110 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588271 | (1 of 1) PF07556 - Protein of unknown function (DUF1538) (DUF1538) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGCAGTGGGCCTGGGCGTGTGTGCAAC |
| Internal bar code: | AAACCCCAGTAGGGGAAAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 99.3437 |
| LEAP-Seq n confirming: | 4541 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAACACACCGATACAGA |
| Suggested primer 2: | TGGGTGCGTATGTAGGTCAA |