| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134164 |
| Chromosome: | chromosome 9 |
| Location: | 3474701 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389504 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCTATGATCTGGGCCGCACGTCCGTA |
| Internal bar code: | GTTGCATCATGATGGAAAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 99.7756 |
| LEAP-Seq n confirming: | 2668 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTACCGCAGCACTGTGT |
| Suggested primer 2: | CACACCTCCCAATGCTCTTT |