Insertion junction: LMJ.RY0402.134195_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.134195
Chromosome:chromosome 2
Location:6057142
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g112701 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCCCGAGCCGCCGCCGTACGACCCATGC
Internal bar code:ATGATACGGCAGGAGAGTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:531
LEAP-Seq percent confirming:78.0142
LEAP-Seq n confirming:550
LEAP-Seq n nonconfirming:155
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGTCTCCAGCTCCTCCA
Suggested primer 2:GTGATGGAGTTCCTGGTCGT