| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134196 |
| Chromosome: | chromosome 7 |
| Location: | 2806702 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331700 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCTCCGGAGCTTCTGGCAATATTAGGT |
| Internal bar code: | AGGCTAATGACCGGTTTGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 64.7355 |
| LEAP-Seq n confirming: | 257 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGGGTCCATGTAAGAGT |
| Suggested primer 2: | CATGCAGAGACAGCCTGGTA |