| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134196 |
| Chromosome: | chromosome 11 |
| Location: | 2780574 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477400 | CYG43 | (1 of 1) PF00072//PF00211//PF02518 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c); Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCCGGACCAGACCAGGTCGGGGCTGTC |
| Internal bar code: | CTCCTGGTACGCCCCTTTACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 99.6626 |
| LEAP-Seq n confirming: | 8271 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTAACTGCATGTCCGACC |
| Suggested primer 2: | GTGACGGCTCTTCTGGAGTC |