| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134209 |
| Chromosome: | chromosome 10 |
| Location: | 1152036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425750 | (1 of 1) PTHR11227:SF18 - WD REPEAT DOMAIN PHOSPHOINOSITIDE-INTERACTING PROTEIN 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTACCGCACACCACTGGTTGTTGGAGA |
| Internal bar code: | AACCTAGTTTTATGAACGACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 99.8159 |
| LEAP-Seq n confirming: | 5423 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATGTGCAGTATCTCGGT |
| Suggested primer 2: | TACTGCAAGTTCATGGAGCG |