Insertion junction: LMJ.RY0402.134254_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.134254
Chromosome:chromosome_8
Location:4184545
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre08.g380151 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGATTTATCATGCATGTAACCCCCTCCCC
Internal bar code:GTATACCGTGATCCCCTTTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTACCCTTGTGTCACGAT
Suggested primer 2:GCTGGACAAGGAGGTGAGAG