Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.134268 |
Chromosome: | chromosome_10 |
Location: | 1741574 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g430700 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTTGGGTTCGGGCGGTACTGGTACTGTGTG |
Internal bar code: | ATCGGTTGGGGCGGTGGCGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 504 |
LEAP-Seq percent confirming: | 98.9358 |
LEAP-Seq n confirming: | 303178 |
LEAP-Seq n nonconfirming: | 3261 |
LEAP-Seq n unique pos: | 251 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATTGTAGTCGGCCCAAAGT |
Suggested primer 2: | ACACACACACACACACACGG |