| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134268 |
| Chromosome: | chromosome 10 |
| Location: | 1741574 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430700 | FAP308 | Flagellar Associated Protein 308 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACACGCGCCCCTGCCCCGCCCGCTCACC |
| Internal bar code: | CACAGGTTTGGCTTGTTGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 722 |
| LEAP-Seq percent confirming: | 99.7028 |
| LEAP-Seq n confirming: | 23480 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGTAGTCGGCCCAAAGT |
| Suggested primer 2: | ACACACACACACACACACGG |