Insertion junction: LMJ.RY0402.134272_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.134272
Chromosome:chromosome 6
Location:6453532
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g292750 (1 of 2) PF05694 - 56kDa selenium binding protein (SBP56) (SBP56) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGTGTGGCAACGATGTTCACGACCTGCC
Internal bar code:TCAACGTGTCCGAACCGGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:681
LEAP-Seq percent confirming:99.5496
LEAP-Seq n confirming:1326
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CAATAACCACCGCACATACG
Suggested primer 2:TATTTCCTTCAATGGGCTGC