Insertion junction: LMJ.RY0402.134295_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.134295
Chromosome:chromosome 6
Location:3678149
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278138 OPR119 OctotricoPeptide Repeat protein 119, pseudogene; (1 of 781) IPR000104 - Antifreeze protein, type I intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCAAAGGTTCGGCGGGAACCGTGCGTGC
Internal bar code:GGGAGGGGCTGGACACGCTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:676
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1797
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTTGTGGATGCAGAAGAT
Suggested primer 2:GGCCATTCAATACCTGACCA