Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.134304 |
Chromosome: | chromosome_3 |
Location: | 2074048 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g156700 | FAP185 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCGACGCAAGGGAATACACAGGTGGCATG |
Internal bar code: | TCCACGAGAATCGTTAGTGCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 204 |
LEAP-Seq percent confirming: | 95.7212 |
LEAP-Seq n confirming: | 6510 |
LEAP-Seq n nonconfirming: | 291 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATCAAGGGACCCCATTTTC |
Suggested primer 2: | GTGGTATTCATTGTTCGGGG |