| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134304 |
| Chromosome: | chromosome 3 |
| Location: | 2074048 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156700 | FAP185,Nphp3 | (1 of 2) IPR011990//IPR013026//IPR019734 - Tetratricopeptide-like helical domain // Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat; Flagellar Associated Protein 185 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGACGCAAGGGAATACACAGGTGGCATG |
| Internal bar code: | TCCACGAGAATCGTTAGTGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 204 |
| LEAP-Seq percent confirming: | 95.7212 |
| LEAP-Seq n confirming: | 6510 |
| LEAP-Seq n nonconfirming: | 291 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCAAGGGACCCCATTTTC |
| Suggested primer 2: | GTGGTATTCATTGTTCGGGG |