Insertion junction: LMJ.RY0402.134304_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.134304
Chromosome:chromosome_3
Location:2074048
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre03.g156700 FAP185 Flagellar Associated Protein antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTCGACGCAAGGGAATACACAGGTGGCATG
Internal bar code:TCCACGAGAATCGTTAGTGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:204
LEAP-Seq percent confirming:95.7212
LEAP-Seq n confirming:6510
LEAP-Seq n nonconfirming:291
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AATCAAGGGACCCCATTTTC
Suggested primer 2:GTGGTATTCATTGTTCGGGG