| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134327 |
| Chromosome: | chromosome 6 |
| Location: | 5875801 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288050 | MGTE1,MGTE | Mg2+/divalent cation transporter; (1 of 2) PF01769 - Divalent cation transporter (MgtE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCATAAAGAAATCAGTGCCGTTCAGCAGC |
| Internal bar code: | CGAACGGTGTAAATGGCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 907 |
| LEAP-Seq percent confirming: | 98.8335 |
| LEAP-Seq n confirming: | 8896 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGACACCCCCTTAAAA |
| Suggested primer 2: | CATGTTCTTGATGCCAAACG |