| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134331 |
| Chromosome: | chromosome 10 |
| Location: | 3269605 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443250 | CCT3 | T-complex protein 1, gamma subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCCTACGTCCGCTGTGCACGCAATGGC |
| Internal bar code: | TTAACGTTTTCGGCAGCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 99.5124 |
| LEAP-Seq n confirming: | 8776 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCCTCTAGCCTCGACCC |
| Suggested primer 2: | CTCTCCCCCTCCTAGTGGTC |