| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134331 |
| Chromosome: | chromosome_10 |
| Location: | 3269608 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g443250 | CCT3 | T-complex protein 1, gamma subunit | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTCTTAACTGGGTTCTTAAGCTCGTGCTG |
| Internal bar code: | CAGAACGGGGCTATCCCAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 734 |
| LEAP-Seq percent confirming: | 98.7308 |
| LEAP-Seq n confirming: | 4823 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTCCTCTAGCCTCGACCC |
| Suggested primer 2: | CTCTCCCCCTCCTAGTGGTC |