| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134335 |
| Chromosome: | chromosome 3 |
| Location: | 5504354 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185800 | (1 of 14) PF00892 - EamA-like transporter family (EamA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTCCGCGCTAGTTGAAATAGTTGAAA |
| Internal bar code: | AGGACAGATCTTTCGGAGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 98.6231 |
| LEAP-Seq n confirming: | 573 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTTCCTCATGATGCTGGG |
| Suggested primer 2: | GTAGGCATAGTGGCCGTTGT |