| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134341 |
| Chromosome: | chromosome 6 |
| Location: | 5040434 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g280385 | PAP6 | predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCACTTCACCTCATGCGCACCGCTGGC |
| Internal bar code: | GGTGATATGGTCGCGCAATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 72.6967 |
| LEAP-Seq n confirming: | 2375 |
| LEAP-Seq n nonconfirming: | 892 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCACAGGTGTGGGTCTC |
| Suggested primer 2: | ACAGATCCAGATCCGTCAGC |