| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134347 |
| Chromosome: | chromosome_7 |
| Location: | 4265041 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g341750 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCTGGCGGTGTCCGACACTGTGGCAGTAGT |
| Internal bar code: | CGACTGGGCAGGATATCGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 251 |
| LEAP-Seq percent confirming: | 95.2663 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGCCTACTGTCATGGTT |
| Suggested primer 2: | AACCAATCACTGCACCAACA |