Insertion junction: LMJ.RY0402.134379_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.134379
Chromosome:chromosome 2
Location:3239205
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095076 ROC39,MFT10 Major facilitator superfamily transporter; (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCCATCCATCCACTGGGTCGTCAGCATC
Internal bar code:TGCAGTTGAATCCCGTTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:330
LEAP-Seq percent confirming:99.4753
LEAP-Seq n confirming:14407
LEAP-Seq n nonconfirming:76
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCCCCTTCATCCTAGAC
Suggested primer 2:GTACACCGCCATTGTGTACG