Insertion junction: LMJ.RY0402.134408_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.134408
Chromosome:chromosome 6
Location:8540466
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g308200 (1 of 2) K09531 - DnaJ homolog subfamily C member 11 (DNAJC11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGTAAAAGGTGATGCGGAAACGCATGCGG
Internal bar code:AGCGAGAAGTCCGCCCGCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:683
LEAP-Seq percent confirming:99.8225
LEAP-Seq n confirming:2812
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGATCGTTTGACCAGTTC
Suggested primer 2:GTAGGGAGGTAGGGAGGTGC