| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134421 |
| Chromosome: | chromosome 12 |
| Location: | 8538727 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547651 | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAGCTGGACTAAGGGGCTAAGTTACCTT |
| Internal bar code: | TTGAGCACGGTTTCGTGCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 99.7148 |
| LEAP-Seq n confirming: | 1049 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAAACGAACAAACCCGTG |
| Suggested primer 2: | GAGCCTCCTGTCGGTATCAC |