Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.134439 |
Chromosome: | chromosome_7 |
Location: | 5523465 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g351150 | FA2 | Flagellar Autonomy Protein, NIMA family kinase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAATAAACTACGCGGCTATGATTGGCAGTT |
Internal bar code: | CGTTTCCATATAAGAGTTCTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 364 |
LEAP-Seq percent confirming: | 99.5032 |
LEAP-Seq n confirming: | 4406 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGTTGTGTGTGAGGTGGAT |
Suggested primer 2: | TCAAGTGACTTCATGGCAGC |