| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134453 |
| Chromosome: | chromosome 6 |
| Location: | 8143381 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g305050 | EEP5,CGL45 | expressed protein Conserved in the Green Lineage; (1 of 90) PF03372 - Endonuclease/Exonuclease/phosphatase family (Exo_endo_phos) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGCAGAGGGTGAGCACGGCGGTGACG |
| Internal bar code: | GCGTGGGCCGGGTGGCAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 445 |
| LEAP-Seq percent confirming: | 97.7941 |
| LEAP-Seq n confirming: | 931 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTCCTACTGCAAAGCCT |
| Suggested primer 2: | CTCGAGCTGCTCATGTTCTG |