| Insertion cassette: | CIB1 | 
| Side of cassette: | 3' | 
| Strand: | - | 
| Strain: | LMJ.RY0402.134457 | 
| Chromosome: | chromosome 3 | 
| Location: | 78832 | 
| Confidence (%): | 95 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre03.g143827 | FBB7 | Flagellar/basal body protein 7; (1 of 1) IPR001680//IPR011047//IPR011048//IPR017986 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // Cytochrome cd1-nitrite reductase-like, haem d1 domain // WD40-repeat-containing domain | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCAACCAAACTTGCCGGACGCTGGCCG | 
| Internal bar code: | TCTCTATGAGGTAACGGGCCAT | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 | 
| LEAP-Seq percent confirming: | 99.5794 | 
| LEAP-Seq n confirming: | 3078 | 
| LEAP-Seq n nonconfirming: | 13 | 
| LEAP-Seq n unique pos: | 25 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGCTGTACGATAAGGTC | 
| Suggested primer 2: | CTTGGCGGTAAAGAGCAGTC |