Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.134457 |
Chromosome: | chromosome_3 |
Location: | 78880 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g143827 | FBB7 | Flagellar/basal body protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCAGATTTAACCCTCGCCCCCTCACCTCGC |
Internal bar code: | CCGTCGACCTCGAGCTCGGTCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 757 |
LEAP-Seq percent confirming: | 99.9364 |
LEAP-Seq n confirming: | 1572 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGGCTGTACGATAAGGTC |
Suggested primer 2: | CTTGGCGGTAAAGAGCAGTC |