| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134471 |
| Chromosome: | chromosome 17 |
| Location: | 2944144 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g719900 | PWD1 | Phosphoglucan water dikinase 1; (1 of 1) K15535 - phosphoglucan, water dikinase (PWD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGACGTTGGCAAGAACGTAGATGGCTAGC |
| Internal bar code: | GGCGCCCATTGTGCGGTTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 942 |
| LEAP-Seq percent confirming: | 99.9118 |
| LEAP-Seq n confirming: | 3397 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGAGGTGGAGAAGGCTA |
| Suggested primer 2: | ATGACAATTTCCGAGGTTCG |