| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134471 |
| Chromosome: | scaffold 18 |
| Location: | 91481 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748597 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGCTCACCTCCCCATGGTCGTCGCCCC |
| Internal bar code: | CCTTCTAGGCGATGTCCTGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 97.6744 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAAAGTGCAAAAGATGGG |
| Suggested primer 2: | CTTTGGGCTGGTATTTCCAA |