| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134474 |
| Chromosome: | chromosome 14 |
| Location: | 3186773 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629102 | (1 of 2) PTHR11731//PTHR11731:SF117 - PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED // GLUTAMYL ENDOPEPTIDASE, CHLOROPLASTIC-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGGTGCGAGGGGGAGGGAGACAGAGGGA |
| Internal bar code: | GCGGACGGCCGCTTTACAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 99.7785 |
| LEAP-Seq n confirming: | 2703 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCAAGAGCAGACAAG |
| Suggested primer 2: | ACCCAACAAGCACTTTCCAC |