| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.134507 |
| Chromosome: | chromosome 16 |
| Location: | 4233096 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668450 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGCATCTCCGCCTCAGAGATCAGACCAG |
| Internal bar code: | GGCACCGTGGAGGCTCGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 92.7582 |
| LEAP-Seq n confirming: | 2946 |
| LEAP-Seq n nonconfirming: | 230 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGTCGCATACCGCATCTG |
| Suggested primer 2: | CGTTGTGGTGAAGTCGGAAT |