| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134532 |
| Chromosome: | chromosome 4 |
| Location: | 2700819 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223350 | CYG62 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTGCTGTTGCTGGCCGCATGCCTGGCG |
| Internal bar code: | CACGCAGAGTCGCCACTGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1173 |
| LEAP-Seq percent confirming: | 99.6095 |
| LEAP-Seq n confirming: | 12500 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTCACAGTCCACACGCT |
| Suggested primer 2: | GGCTACAGGCTTGACAGAGG |