| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134542 |
| Chromosome: | chromosome 16 |
| Location: | 7483661 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687182 | POB10 | Proteome of basal body 10; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCGACATGTGGGTTTGGCAGCGCGTAT |
| Internal bar code: | ACTGACTCTCGTGCCGCGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 468 |
| LEAP-Seq percent confirming: | 99.8624 |
| LEAP-Seq n confirming: | 1452 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCATCCAACAGTGCAAGA |
| Suggested primer 2: | CGGTGCTTTCTCTCAGGTTC |