| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.134550 |
| Chromosome: | chromosome 4 |
| Location: | 1192131 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215500 | (1 of 1) PF09735 - Membrane-associated apoptosis protein (Nckap1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGAAGAGCCTGGATGCGCGGGCGCCCA |
| Internal bar code: | TGCAAATTATGTCGTAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 323 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 125 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTCTTCCAGTAGCAGGC |
| Suggested primer 2: | AGTATGGCTGCTCAAAGCGT |