| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.134563 |
| Chromosome: | chromosome 7 |
| Location: | 4676464 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344668 | (1 of 2) IPR000104//IPR004827 - Antifreeze protein, type I // Basic-leucine zipper domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGAGATGTTTCCTTTCCTGTGGGTGT |
| Internal bar code: | GGCTCCCGGTACCGCGGCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 99.5086 |
| LEAP-Seq n confirming: | 405 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCCTATTCACGCGTTTC |
| Suggested primer 2: | GTTTGCATTTGTGTGATGCC |