| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.134576 |
| Chromosome: | chromosome 2 |
| Location: | 5763233 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110600 | DEG5 | (1 of 2) PTHR22939:SF99 - PROTEASE DO-LIKE 8, CHLOROPLASTIC; Deg protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGCTGCCGCGTGCCTGTACACATCAGG |
| Internal bar code: | ATTCCAGCACACCATTACGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.8118 |
| LEAP-Seq n confirming: | 5833 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAACGGCCAAACATCTGGT |
| Suggested primer 2: | CCCCTTACGGCACACATAGT |